Fig. 4

Chromatin immunoprecipitation (ChIP) sequencing fragment analysis and sample distribution. To measure the distance between subpeaks and find the maximum correlation, the cross-correlation function (CCF) was used to assess a H3K4me3 (248 bp) and (b) H3K27me3 (247 bp). Multi-dimensional scaling (MDS) plots indicate the log fold change (logFC) between samples within the (c) H3K4me3 and (d) H3K27me3 groups, describing sample-to-sample distances. Representative histone peaks are shown for differential binding regions (P ≤ 0.05) for both (e) H3K4me3 and (f) H3K27me3. Con = control, Exp = maternal nano-TiO2 exposed, H3K4me3 = histone 3 lysine 4 tri-methylation, H3K27me3 = histone 3 lysine 27 tri-methylation, Wnt5a = Wnt Family Member 5A, Rn5-8 s = 5.8S ribosomal RNA for Rattus norvegicus