Fig. 2

O-PMs-increased ICAM-1 Expression in A549 Cells, but not W-PMs. a-b A549 cells were exposed to 0, 25, 50 or 100 μg/ml O-PMs (a) or W-PMs (b) for 24 h. ICAM-1 protein in cell lysates was measured by Western blot as described in the Methods section. GAPDH was used as the loading control. *p < 0.05 vs. untreated cells. †p < 0.05 vs. O-PMs-25. #p < 0.05 vs. O-PMs-50. c The distribution of ICAM-1 was analyzed by immunofluorescent staining. Bar = 100 mu. d-e A549 cells were pretreated with 5 mM NAC for 1 h and then treated with 100 μg/ml O-PMs for 24 h. ICAM-1 protein in cell lysates was measured by Western blot (d) and the ICAM-1 surface expression was detected by flow cytometer (e). *p < 0.05 vs. Con. †p < 0.05 vs. O-PMs. f Representative fluorescence photomicrographs and quantitative data showing the effect of ICAM-1 expression on the adhesion of BCECF-AM-labeled U937 cells to O-PMs-treated A549 cells. A549 cells were cotreated with 100 μg/ml O-PMs and with 1 μg/ml anti-ICAM-1 antibody or anti-IgG for 24 h. Bar = 125 mu. *p < 0.05 vs. Con. †p < 0.05 vs. O-PMs