Fig. 5

IL-6 was involved in O-PMs-induced ICAM-1 expression. a Representative photographs of the cytokine antibody array hybridized with conditioned media from A549 cells with or without 100 μg/ml O-PMs treatment for 24 h. b A549 cells were exposed to 0, 25, 50, or 100 μg/ml O-PMs for 24 H. IL-6 protein in cell lysates was measured by Western blot as described in the Methods section. GAPDH was used as the loading control. c A549 cells were pretreated with 20 μg/ml TCZ or with 5 μg/ml of sgp130Fc or with IL-6 siRNA, respectively, and then with treated with 100 μg/ml O-PMs for 24 H. IL-6 concentration in conditioned media was determined by ELISA. *p < 0.05 vs. Con. †p < 0.05 vs. O-PMs. d-e The effects of TCZ (d) and IL-6 (e) on the expression of ICAM-1 expression and the phosphorylation of AKT, p65 and STAT3. *p < 0.05 vs. the untreated cells. †p < 0.05 vs. O-PMs