| |
Concentration (μg/ml)
|
Log EC50 (M)
|
Emax (%)
|
|---|
|
ACh
|
NTG
|
5-HT
|
ACh
|
NTG
|
5-HT
|
|---|
|
Control
|
0
|
−6.1 (− 6.3 to − 5.8)
|
− 6.6 (− 7.0 to − 5.7)
|
− 5.3 (− 5.5 to − 5.2)
|
42.9 (38.7–48.6)
|
78.3 (66.7–113.2)
|
115.8 (105.1–127.5)
|
|
E171
|
14
|
−5.9 (− 6.1 to − 5.7)
|
− 6.9 (− 7.1 to − 6.6)
|
− 5.4 (− 5.6 to − 5.2)
|
56.3* (52.5–61.3)
|
65.3 (59.9–73.5)
|
148.5* (131.3–168.2)
|
|
140
|
−6.1 (− 6.4 to − 5.6)
|
− 7.1 (− 7.4 to − 6.7)
|
− 5.3 (− 5.5 to − 5.1)
|
44.9 (38.9–55.2)
|
67.2 (60.4–79.3)
|
172.7* (151.4–197.3)
|
|
Aeroxide P25
|
14
|
−6.1 (− 6.4 to − 5.8)
|
−6.9 (− 7.3 to − 6.1)
|
− 5.3 (− 5.6 to − 5.1)
|
45.9 (41.8–51.8)
|
70.8 (61.0–98.8)
|
130.6 (111.3–152.5)
|
|
140
|
−5.7 (− 6.0 to − 5.3)
|
− 6.8 (− 7.0 to − 6.6)
|
−5.4 (− 5.5 to − 5.3)
|
42.4 (37.7–51.4)
|
80.5 (73.7–91.3)
|
122.5 (114.0–131.4)
|
|
E153
|
10
|
−6.0 (− 6.2 to − 5.7)
|
−6.8 (− 7.1 to − 6.2)
|
−5.4 (− 5.7 to − 5.1)
|
50.4 (45.9–57.5)
|
61.0 (53.3–79.6)
|
147.0 (122.7–179.5)
|
|
100
|
−5.9 (− 6.1 to − 5.7)
|
− 6.7 (− 7.3 to 0.2)
|
−5.1 (− 5.3 to − 4.9)
|
57.5* (52.7–64.6)
|
84.1 (67.5–514.0)
|
143.4 (125.7–166.5)
|
|
Printex 90
|
10
|
−6.1 (− 6.7 to − 2.1)
|
−6.0 (− 6.5 to − 3.4)
|
− 5.3 (ND to − 5.2)
|
48.7 (39.3–161.2)
|
68.7 (53.0–188.8)
|
147.1* (134.8–160.0)
|
|
100
|
−6.0 (− 6.3 to − 5.6)
|
−6.4 (− 6.8 to − 5.6)
|
−5.3 (− 5.5 to − 5.2)
|
50.8 (45.3–59.3)
|
75.6 (63.9–109.5)
|
123.6 (112.5–135.4)
|
- The concentration-response curves were performed for acetylcholine (ACh), nitroglycerine (NTG) and 5-hydroxytryptamine (5-HT) on aorta from rats exposed ex vivo to E171, Aeroxide P25, E153 or Printex 90 for 30 min. The data are presented as the mean and 95% CI. The number of animals in each group varied; specified in Figs. 3, 4 and 5. Asterisk (*) denotes statistically significant effects on Emax when compared to the effect in the control group (P < 0.05), ND: the value could not be determined by non-linear regression analysis
