Fig. 3
From: Carbon black suppresses the osteogenesis of mesenchymal stem cells: the role of mitochondria
Effects of Printex 90 on MSCs viability. a TEM images of MSCs uptaking Printex 90. Untreated cells (Control) showed no Printex 90 inside of cells whereas Printex 90-treated MSCs (CB- treatment) showed the presence of Printex 90 (shown at the arrow) in the cytoplasm but no in the nuclear. N: nucleus; M: mitochondria. Scale bar in normal graphs = 1 μm and in graphs with black box =200 nm. MSCs were treated with Printex 90 at indicated concentration for 24 h (b) or 7 d (c). After treatment, CCK-8 reagent was added to the culture and incubated for another 2 h and the absorbance was recorded at 450 nm