Fig. 4

Effects of Printex 90 on osteogenesis of MSCs. MSCs were treated by Printex 90 at indicated concentration and induced to osteogenic differentiation for 7 d. After treatment, mRNA and proteins were collected for RT-PCR analysis (a) and Western blot (b), respectively. c The quantification of Western blot. Printex 90 treated cells were induced to osteogenic differentiation for 10 d followed by ALP activity (d) or ALP staining (e). (f) Alizarin Red S staining showed the mineralization nodules after osteogenic induction for 21 d. Printex 90 treated cells were induced to osteogenic differentiation for 21 d. Cells were fixed and stained by Alizarin Red S. Images were collected with 10× magnification of the light microscopy. Bar = 500 μm