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Fig. 6 | Particle and Fibre Toxicology

Fig. 6

From: Carbon black suppresses the osteogenesis of mesenchymal stem cells: the role of mitochondria

Fig. 6

Effects of Printex 90 on mitochondrial functions in the process of MSCs osteogenic differentiation. MSCs were treated with 3 ng/mL and 30 ng/mL Printex 90 and induced to osteogenic differentiation within 7 d. a Reduced ATP content was found in CB Printex 90-treated cells when compared with control after osteo-induction for 7 d. b-c XF-96 Flux Analyzer showed the decreased basal oxygen consumption rate (OCR), ATP-linked respiration, proton leak, the maximal OCR, spare respiratory capacity (SRC) and non-mitochondrial respiration after osteo-induction for 4 d. d Western blotting showed the protein expression of five mitochondrial oxidative phosphorylation (OXPHOS) complex subunits after osteo-induction for 7 d. e Quantification of the Western blots. f Representative image from flow cytometer with cells stained by JC-10 probe after osteo-induction for 7 d. g The relative red/green ratio of cells stained by JC-10 probe

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