Fig. 7
From: Carbon black suppresses the osteogenesis of mesenchymal stem cells: the role of mitochondria
Impairment of mitochondrial biogenesis, dynamics and mitophagy. MSCs were treated with 3Â ng/mL and 30Â ng/mL Printex 90 and induced to osteogenic differentiation for 7 d. a RT-PCR demonstrated the lower expression of mtDNA, including COX1 and ND1 after osteo-induction for 7 d. After treatment for 7 d, mRNA or proteins were isolated for RT-PCR (b)&(e) and Western blot (c)&(f)&(h). (d)&(g)&(i) The quantification of Western blots