Fig. 3From: Radical containing combustion derived particulate matter enhance pulmonary Th17 inflammation via the aryl hydrocarbon receptorMCP230 exposure activates aryl hydrocarbon receptor (AHR) and increases the expression of Cyp1a1. (a) Activation of AHR as measured using a dual AHR luciferase reporter assay in A549 cells. A549 cells were exposed to MCP230 (50 μg/cm2) or TCDD (50 nM) and simultaneously treated with or without trolox (antioxidant; 100 μM) for 4 h. In addition, AHR activation was assessed in cells exposed to non-EPFR-containing particle controls such as SiO2, CuO/SiO2, and MCP50 (50 μg/cm2). AHR promoter activity is expressed as normalized luminescence using a Renilla reporter for internal normalization. Data represent mean ± SEM from one of two independent experiments, performed in triplicate. ap < 0.05, compared to MCP50 group. bp < 0.05, compared to MCP230 group, one-way ANOVA with Dunnett’s multiple comparisons test. (b) Activation of AHR as measured by Cyp1a1 and Cyp1b1 expression relative to Gapdh using RT-qPCR analysis in the bone marrow-derived dendritic cells (BMDCs). BMDCs from WT and Ahr−/− mice were exposed to various concentrations of MCP230 for 4 h. Data represent mean ± SEM from one of two independent experiments, performed in duplicate. ap < 0.05, compared to WT (0 μg/cm2) group. bp < 0.05, compared to WT (12.5 μg/cm2) group, one-way ANOVA with Tukey’s multiple comparisons testBack to article page