Fig. 6

Acute exposure to MCP230 results in transient activation of AHR and increased expression of Cyp1a1 and Cyp1b1 in vivo. (a, b) WT mice were exposed to vehicle or MCP230 (50 μg) and lungs were isolated at 4 and 24 h post-exposure. Expression of Cyp1a1 and Cyp1b1 relative to Hprt was measured using RT-qPCR analysis. Data represent mean ± SEM from one of two independent experiments. ^ap < 0.05, compared to Vehicle group. ^bp < 0.05, compared to 4 h group, one-way ANOVA with Tukey’s multiple comparisons test. (c) Representative photomicrographs of lungs from WT mice exposed to vehicle or 50 μg MCP230. Lungs were isolated from mice at 4 h post-exposure and stained for Cyp1a1 RNA using in situ hybridization. Top panel represents expression of Cyp1a1 in lung parenchyma and bottom panel represents expression of Cyp1a1 in the airway epithelium. Arrowheads point at cells expressing Cyp1a1