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Table 3 Morphological transformation versus outcome in genotoxicity assays in Syrian hamster Embryo (SHE) cells treated with different forms of amorphous and crystalline silica

From: An updated review of the genotoxicity of respirable crystalline silica

Test material Dose (μg/cm2) MN1
(% of cells)
24 h
Comet1
(% tail)
+ FPG, 24 h
Morphological transformation frequency (%)
Control 0 2.3 ± 0.5 13.0 ± 1.0 0.01
Diatomaceous earth (DE)
(GSD: 1.35 μm)
3.81    0.02
7.62    0.02
11.4   11.0 ± 1.2  
13.6 2.1 ± 0.8   
15.24    0.11*
22.8   18.0 ± 2.0  
27.2 2.0 ± 0.6   
30.48    0
45.7   21.0 ± 2.5 *  
54.4 1.8 ± 0.6   
Heated DE (see above), containing 47% Cristobalite
(GSD: 4.85 μm)
3.81    0.16*
7.62    0.19*
11.4   6.5 ± 1.5  
13.6 3.0 ± 1.0   
15.24    0.23*
22.75 2.0 ± 0.6 9.0 ± 1.0  
30.48    0.4*
45.7   10.0 ± 4.5  
54.4 1.8 ± 0.3   
Quartz Min-U-Sil 5
(GSD: 1.33 μm)
3.81    0.24*
7.62    0.17*
11.4   9.8 ± 1.2  
13.6 2.2 ± 1.3   
15.24    0.71*
22.8   11.0 ± 2.7  
27.75 2.2 ± 1.3   
30.48    0.77*
45.7   8.0 ± 1.0  
54.4 1.5 ± 0.3   
  1. 1Data were estimated from graphical representations in Darne et al [24] (Figures. two A (MN) and Figures. three B (Comet), reproduced with permission). GSD Geometric mean diameter
  2. * = statistically significant (p < 0.05) compared to control