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Fig. 5 | Particle and Fibre Toxicology

Fig. 5

From: Cerium dioxide nanoparticles exacerbate house dust mite induced type II airway inflammation

Fig. 5

CeO2NPs and HDM alter airway mucin and marker expression after repeat exposure. Mice (n = 6–7 per treatment group) were exposed to CeO2NPs at either low dose (CeLD) (75 μg/kg) or high dose (CeHD) (750 μg/kg) with and without HDM (1.25 mg/kg), according to instillation protocols described in Fig. 1. Lung tissue was fixed and sections processed for PAS staining (a; Top panels) or immunohistochemical detection of CLCA1 protein (a; Bottom panels). Representative images are displayed with a scale bar of 100 μm. Quantification of airway mucin positive cells was also carried out and displayed as mean ± SEM PAS score (b). Total lung mRNA was also examined for mucin related gene expression by RT-PCR analysis (C-E) and results expressed as mean ± SEM fold over control (F.O.C.) levels. Statistical significance between treatments was assessed using one way ANOVA. Comparisons between particle and HDM treatments alone and control levels are represented as (* p < 0.05), while comparisons between particle + HDM combinations and HDM levels are represented as (# p < 0.05)

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