Fig. 6
Chromosomal damage and rearrangements. Micronuclei in binucleated cells (a), micronuclei in mononucleated cells (b), nucleoplasmic bridges (c) and nuclear buds (d) frequencies as evaluated by CBMN Cyt assay following 48 h exposure of BEAS-2B cells to Ni, NiO or NiCl2 (μg Ni/mL). Results are presented as mean ± SEM from three independent experiments (n = 3) of the different kind of damage in 1000 binucleated or mononucleated cells. Mitomycin C (MMC, 0.05 μg/mL) was used as positive control. *, p < 0.05; **, p < 0.01; ***, p < 0.001