Fig. 7
Effect of iron chelator and calcium modulators on chromosomal damage and rearrangements. Micronuclei in binucleated cells (a), micronuclei in mononucleated cells (b), nucleoplasmic bridges (c) and nuclear buds (d) frequencies as evaluated by CBMN Cyt assay following co-exposures of 5 μg Ni/mL Ni, NiO or NiCl2 with deferoxamine, verapamil, dantrolene or BAPTA-AM for 48 h. Results are presented as mean ± SEM from three independent experiments (n = 3) of the different kind of damage in 1000 binucleated or mononucleated cells. *, p < 0.05; **, p < 0.01; ***, p < 0.001. Asterisks represent the statistical significance compared to the control whereas a, b and c represent the significance of the chelator/modulator compared to Ni-, NiO-, NiCl2- treated cells, respectively