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Fig. 7 | Particle and Fibre Toxicology

Fig. 7

From: Effects of differently shaped TiO2NPs (nanospheres, nanorods and nanowires) on the in vitro model (Caco-2/HT29) of the intestinal barrier

Fig. 7

Three-dimensional confocal images of the Caco-2/HT29 co-culture barriers z-scans from Fig. 6. Images were taken after exposures of 24 or 48 h to TiO2NPs-S (a and b), TiO2NPs-R (c and d), and TiO2NPs-W (e and f). Cell nuclei (blue) were stained with Hoechst, and mucus (red) was stained with WGA. NPs were visualized by reflection and marked with a green mask. White arrows indicate NPs in the cell cytoplasm and NPs-nucleus interactions. Images were processed with the Imaris 7.2.1 software

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