Table 1 Genes encoding molecular markers of the Caco-2/HT29 barrier, and analysed by RT-qPCR
Gene Identification | Encoded protein name | Function |
|---|---|---|
ALPI | Intestinal alkaline phosphatase | Digestive brush-border enzyme. Detoxification of lipopolysaccharides |
SI | Sucrase-isomaltase | Digestion of dietary carbohydrates including starch, sucrase and isomaltase |
SLC15A1 | Solute carrier family 15 member 1 | Intestinal hydrogen peptide cotransporter. Uptake of di- and tri-peptides from the lumen and into enterocytes |
ZO-1 | Zonula occludens-1 | Tight junction adaptor protein that also regulates adherent junctions. |
OCLN | Occludin | Integral membrane protein. Required for cytokine-induced regulation of the tight junction paracellular permeability barrier. |
CLDN2 | Claudin-2 | Claudin proteins are identified as major integral membrane proteins, localized exclusively at tight junctions in the intestine. |