Fig. 1
From: Differential effects of diesel exhaust particles on T cell differentiation and autoimmune disease
EAE Methods. Starting on day − 12 mice every 3 days until day 9, age-matched female mice receive intranasal treatment of SRM1650b PM or OF, SRM2975 PM or OF, and corresponding solvent or PBS control. The mice received 12.5 mg/mL PM or 250 μg PM in 20uL for each dose. On day 0, prior to injections, mice were weighed. A subcutaneous injection of 100 μL MOG35–55 emulsion was given between the shoulder blades of each mouse and an intraperitoneal injection of 200 ng/mouse pertussis toxin in 500 μL was also given. The emulsion contained 50 μg/mouse MOG35–55 in CFA and 200 μg/mouse heat-killed M. tuberculosis. Additionally, on day 2 mice received 200 ng/mouse pertussis toxin in 500 μL intraperitoneally. Mice were scored for clinical symptoms and weighed daily starting on day 7 until day 28 when the mice were sacrificed. Abbreviations: EAE, experimental autoimmune encephalomyelitis; SRM, standard reference materials; PM, particulate matter; OF, organic fraction; PBS, phosphate buffered saline; MOG, myelin oligodendrocyte glycoprotein