Fig. 3

Organic fraction of diesel exhaust particles have differential effects on severity of EAE in vivo. Age matched female C57BL/6 J were exposed intranasally to 12.5 mg/mL PM of SRM1650b OF, SRM2975 OF, or PBS control. The mice were dosed intranasal 8 times starting at day − 12 and continuing every 3 days until day 9. Mice were scored daily for severity of disease. a SRM1650b OF had no effect on severity of EAE. b SRM2975 OF worsens severity of EAE. c SRM1650b OF does not increase peak clinical score. d SRM2975 OF significantly increased peak clinical score. e SRM1650b OF had no effect on cumulative clinical score. (F) SRM2975 OF significantly increased cumulative clinical score over the 28-day period. g, h Neither DEP OF influenced day of disease onset which represents the first day of a clinical score greater than 0. Results are mean ± SEM of (Solvent n = 10), (SRM1650b OF n = 10), and (SRM2975 OF n = 14). Significant differences among groups (p < 0.05) are indicated by an asterisk. Abbreviations: SRM, standard reference materials; PM, particulate matter; OF, organic fraction; PBS, phosphate buffered saline; EAE, experimental autoimmune encephalomyelitis; DEP, diesel exhaust particles