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Fig. 3 | Particle and Fibre Toxicology

Fig. 3

From: Maternal inhalation of carbon black nanoparticles induces neurodevelopmental changes in mouse offspring

Fig. 3

Maternal exposure to Printex 90 carbon nanoparticles affects astrocytes and perivascular macrophages (PVMs) in the offspring cerebral cortex (n = 4–6). a-b show representative light micrographs of glial fibrillary acidic protein (GFAP)-positive astrocytes and PVMs in the cerebral cortices of 6 week old male offspring in the control (a-c, j), low exposure (d-f), and high exposure groups (g-i, k, l). a, d and g gives an overview of the cerebral cortex, with b, e, and h providing enlarged views hereof, respectively, which is further enlarged in c, f, and i, respectively. These enlarged panels were taken from the same location in all brains. c, f, and i show that the expression levels of GFAP around blood vessels are dose-dependently increased by maternal exposure to carbon black nanoparticles. j and k show representative light micrographs of granules in PVMs of 6 week old male offspring in the control and high exposure groups, respectively. l is an enlarged view of panel k. White arrows indicate normal granules and black arrow indicates enlarged granules of PVMs. l shows that the granules in PVMs next to GFAP positive astrocytes are enlarged. Enlarged granules represent a typical histopathological finding following denaturation of PVMs

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