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Table 1 Overall design and group sizes for the different outcomes

From: Maternal inhalation of carbon black nanoparticles induces neurodevelopmental changes in mouse offspring

Group Control Low High
Mass concentration (mg/m3) Below detection limit 4.79 ± 1.86 33.87 ± 14.77
Particle number conc. (cm−3) 4 ± 4 3.59 ± 2.48∙105 2.12 ± 0.69∙106
Exposure period, all females 4–18 4–18 4–18
Exposed time-mated females 20 20 20
Dams delivering littersa 14 16 15
Litter size 10.9 ± 3.2 11.3 ± 3.3 11.1 ± 2.7
Differential cell counts
 - Non-littering females (PND 9) 4 4 5
 - Littering females (PND 26–27)b 5 9 13
Saa3 in lung tissue    
 - Non-delivering females (PND 9) 6 4 5
 - Delivering females (PND 26–27) 11 12 13
Offspring outcomes+
 - GFAP in brain tissue (6 weeks) 6 males 5 males 4 males
 - PV+ interneurons (PND 25)c 6 females and 5 males 7 females and 3 males
 - PV+ interneurons (PND 120)d 6 females 6 females
 - Open field females/malesd 9/11 11/10 13/13
  1. aFemales that did not deliver a litter was killed at PND 9 and inflammatory outcomes assessed. bFor differential cell counts, some slides were accidentally processed with too few cells and could not be included in the analysis, resulting in small group sizes. aA maximum of one male and one female was used in any one test. cThe density of PV+ interneurons and expression of PV were similar for males and females within each condition and were combined prior to statistical analysis. dThe females assessed for PV+ interneurons had been tested in the open field at three months of age