Table 5 Method accuracy and coefficient of variation (CV) for quantification of 8-oxodGuo, 1,N2-εdGuo and 1,N6-εdAdo in DNA
Basal level | Added | Detected | Detected(−)Basal | Accuracy | CV |
|---|---|---|---|---|---|
Average ± SD (fmol) | fmol | Average ± SD (fmol) | Average (fmol) | % | % |
8-oxodGuo | |||||
373.00 ± 2.71 | 0 | 372.79 ± 50,60 | – | – | 13.57 |
373.98 ± 4.86 | 367 | 755.41 ± 107,92 | 381 | 103.93 | 14.29 |
374.84 ± 5.19 | 734 | 1069.57 ± 108,51 | 695 | 94.65 | 10.14 |
357.94 ± 15.05 | 1469 | 1671.67 ± 44,27 | 1314 | 89.43 | 2.65 |
371.07 ± 2.43 | 2204 | 2272.01 ± 40,20 | 1901 | 86.25 | 1.77 |
1,N2-εdGuo | |||||
0.54 ± 0.01 | 0 | 0.54 ± 0.09 | – | – | 16.88 |
0.54 ± 0.01 | 1 | 1.47 ± 0.16 | 0.93 | 93.39 | 11.17 |
0.55 ± 0.01 | 5 | 5.30 ± 0.72 | 4.76 | 95.11 | 13.50 |
0.53 ± 0.01 | 10 | 10.60 ± 0.39 | 10.06 | 100.63 | 3.67 |
0.54 ± 0.01 | 20 | 20.20 ± 0.93 | 19.66 | 98.29 | 4.60 |
1,N6-εdAdo | |||||
2.08 ± 0.10 | 0 | 2.29 ± 0.39 | – | – | 17.05 |
2.05 ± 0.04 | 1 | 3.06 ± 0.47 | 1.01 | 100.89 | 15.31 |
1.99 ± 0.06 | 5 | 7.87 ± 1.66 | 5.88 | 117.60 | 21.10 |
2.03 ± 0.07 | 10 | 12.43 ± 1.25 | 10.41 | 104.06 | 10.06 |
1.97 ± 0.03 | 20 | 22.42 ± 3.89 | 20.46 | 102.29 | 17.34 |