Fig. 6

Effect of AG-QDs on nuclear viability and area as detected by high content screening. a: High content screening images of nucleus (as stained by Hoechst 33342). The NR8383 cells were exposed to AG-QDs (0, 50, 100 and 200 μg/mL) for 24 h before staining and imaging. The stained cell at the bottom right corner is magnified from the cell marked with red box. b Viability (red) and area (blue) of nuclei as obtained from quantitative analysis of the intensity and area of nuclei fluorescence in panel (a). Significant differences among different AG-QDs concentrations (0–200 μg/mL) is marked with different letters (p < 0.05, LSD, n = 6)