Fig. 1

Time course of cell associated silver amounts in RAW 264.7 cells (circle) and bone marrow derived macrophages from wild-type mice (up triangle) and SR-A deficient mice (down triangle) exposed to a nominal concentration of 12.5 μg/mL of 20 nm (a-b) or 110 nm (c-d) silver nanoparticles. RAW 264.7 cells were exposed to measured exposure concentration of 9.15 μg/ml 110 nm silver nanoparticles (c). Solid lines are unmodified ISD3 simulations of silver amounts in cells from measured exposures. The dotted line is the cellular silver content calculated using ISD3 adjusted for reduced uptake of particles reaching SR-A deficient cells exposed to 20 nm silver nanoparticles