Fig. 5

MWCNT High-COOH nanoparticles exacerbate DNFB induced MHCII skin cell expression and granulocyte infiltration. Mice were sensitized with 30 μl 0.05% DNFB in a 4:1 acetone/olive oil vehicle on the abdomen on day 0. On day 5 the mice were collared to prevent grooming and challenged with 50 μl of either vehicle, 5 μg MWCNT, 0.2% DNFB, or 0.2% DNFB with 5 μg MWCNT on a 5 cm² area of dorsal skin for 24 h. A 2 cm² section of skin was processed into a single cell suspension and stained for flow cytometry. The cell populations were gated into total APCs (MHCII+) (a), granulocytes (CD11b+, F4/80-, SSC high) (b), macrophages (MHCII+, F4/80+, CD11c-) (c), and Langerhans cells (MHCII+, F4/80-, CD11c+) (d). The graphs represent the mean percentage of positive cells in the total live cell population (+/− SEM), N = 6. Significance is defined at a p-value < 0.05. * indicates significance compared to control, # indicate significance within groups