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Table 6 BAL fluid cell composition on day 1, 28 and 90 post-exposure

From: Airport emission particles: exposure characterization and toxicity following intratracheal instillation in mice

  Total cell count Neutrophils Macrophages Eosinophils Lymphocytes Epithelial cells
Day 1       
 Vehicle control 56.43 ± 6.42 2.84 ± 0.89 46.58 ± 6.00 1.16 ± 0.79 0.84 ± 0.47 5.00 ± 1.59
  CB 54 μg 144.80 ± 16.23(****) 100.11 ± 11.85(****) 28.52 ± 3.05 9.96 ± 2.58(***) 1.43 ± 0.43 3.85 ± 0.78
  CAP 6 μg 53.32 ± 9.87 6.43 ± 0.88 42.62 ± 8.90 0.30 ± 0.07 0.04 ± 0.04 3.94 ± 1.65
  CAP 18 μg 82.22 ± 11.96 36.72 ± 10.00(***)(¤) 38.28 ± 2.94 1.40 ± 0.42 0.33 ± 0.15 5.49 ± 1.60
  CAP 54 μg 147.50 ± 10.64(****) (¤¤¤¤)(“’) 101.09 ± 11.07(****) (¤¤¤¤)(“’) 38.79 ± 5.78 1.85 ± 0.58(*) (“) 0.67 ± 0.30 5.10 ± 1.24
  JEP 6 μg 66.37 ± 21.58 6.29 ± 3.00 43.83 ± 7.92 10.01 ± 9.69 1.46 ± 1.26 4.77 ± 1.40
  JEP 18 μg 91.02 ± 9.67 25.89 ± 8.57(*) 57.40 ± 3.43(xx) 1.58 ± 0.63 1.01 ± 0.38 5.15 ± 1.40
  JEP 54 μg 160.50 ± 17.40(****)
(¤¤¤¤)(“’)
110.88 ± 14.66(****)
(¤¤¤¤)(“’)
37.40 ± 6.94 4.27 ± 1.75(*)
(“)
3.31 ± 1.05(¤)
(“’)
4.65 ± 0.97
  NIST2975 18 μg 47.92 ± 7.36 1.67 ± 0.46 42.05 ± 7.23 0.17 ± 0.09 0.19 ± 0.12 2.68 ± 0.51
  NIST2975 54 μg 61.50 ± 9.22 25.57 ± 5.82(*) 31.28 ± 3.61 1.05 ± 0.41 0.93 ± 0.27 3.48 ± 0.75
  NIST2975 162 μg 191.33 ± 11.98(****) 148.46 ± 9.74(****) 32.55 ± 4.13 5.07 ± 2.26 1.77 ± 0.59 4.78 ± 0.93
  NIST1650 a 18 μg 87.55 ± 7.706 10.94 ± 2.78 62.91 ± 4.77 0.36 ± 0.13 0.09 ± 0.09  
  NIST1650 a 54 μg 72.238 ± 8.993 12.07 ± 5.46 50.56 ± 3.23 1.03 ± 0.98 0.10 ± 0.07  
  NIST1650 a 162 μg 177.375 ± 16.756 120.54 ± 11.80 48.92 ± 5.75 1.23 ± 0.49 0.21 ± 0.15  
Day 28       
 Vehicle control 51.10 ± 3.89 0.10 ± 0.05 47.09 ± 3.89 0.16 ± 0.08 0.19 ± 0.08 3.55 ± 0.75
  CB 54 μg 75.63 ± 13.40 1.68 ± 0.75(*) 66.83 ± 12.12 0.04 ± 0.04 3.20 ± 0.77(*) 3.88 ± 0.55
  CAP 6 μg 50.20 ± 7.92 1.19 ± 0.84 42.50 ± 5.05 2.82 ± 2.64 1.03 ± 0.73 2.66 ± 0.88
  CAP 18 μg 60.23 ± 8.02 1.05 ± 0.68 46.83 ± 2.83 7.90 ± 5.98 1.43 ± 0.80 3.02 ± 0.65
  CAP 54 μg 48.85 ± 9.20 0.10 ± 0.07 39.18 ± 6.33 3.90 ± 2.76 2.42 ± 1.15(*) 3.25 ± 0.90
  JEP 6 μg 51.47 ± 11.08 0.13 ± 0.08 49.07 ± 10.53 0.02 ± 0.02 0.26 ± 0.09 2.00 ± 0.64
  JEP 18 μg 61.75 ± 7.01 0.27 ± 0.12 63.01 ± 4.84 0.23 ± 0.17 0.59 ± 0.25 2.94 ± 0.72
  JEP 54 μg 46.43 ± 8.56 0.68 ± 0.38(*) 40.18 ± 7.73 0.14 ± 0.07 0.87 ± 0.37(*) 4.56 ± 1.37
  NIST2975 18 μg 60.67 ± 7.71 0.27 ± 0.19 54.98 ± 7.61 0.09 ± 0.06 0.47 ± 0.11 4.87 ± 0.88
  NIST2975 54 μg 50.37 ± 6.07 0.21 ± 0.18 43.35 ± 6.67 0.08 ± 0.05 0.45 ± 0.27 6.27 ± 1.88
  NIST2975 162 μg 81.85 ± 8.40(*) 2.14 ± 0.92(**) 70.53 ± 7.43 0.30 ± 0.30 4.12 ± 1.93(**) 4.75 ± 0.43
  NIST1650a 18 μg 61.01 ± 3.13 0.44 ± 0.22 46.46 ± 2.98 0.11 ± 0.05 0.60 ± 0.12  
  NIST1650 a 54 μg 58.26 ± 7.56 0.18 ± 0.07 45.83 ± 5.68 1.33 ± 0.78 1.27 ± 0.54  
  NIST1650 a 162 μg 83.94 ± 10.64 1.86 ± 0.83 61.86 ± 6.29 0.28 ± 0.12 4.01 ± 1.25  
Day 90       
 Vehicle control 54.03 ± 5.14 0.45 ± 0.16 45.23 ± 4.39 0.98 ± 0.91 3.57 ± 3.39 3.80 ± 0.74
  CB 54 μg 86.93 ± 8.78(**) 2.07 ± 0.49(**) 73.33 ± 6.72 0.09 ± 0.09 4.70 ± 1.79 6.75 ± 1.58
  CAP 54 μg 62.75 ± 4.30 0.92 ± 0.33 56.68 ± 4.52 0.10 ± 0.10 1.30 ± 0.82 3.74 ± 0.39
  JEP 54 μg 50.90 ± 7.07 0.92 ± 0.47 42.69 ± 5.78 0.14 ± 0.07 1.74 ± 1.40 5.42 ± 1.51
  NIST2975 162 μg 48.42 ± 7.09 0.46 ± 0.18 45.11 ± 6.94 0.00 ± 0.00 0.38 ± 0.21 2.47 ± 0.44
  1. aNIST1650 data was included for comparison and obtained from a previously published study (Kyjovska et al. Mutagenesis 2015)
  2. P-value summary: (*) – (****) = p < 0.05 - p < 0.0001 increase compared to vehicle control, (x) – (xxxx) = p < 0.05 - p < 0.0001 increase compared to CB 54 μg, (¤) – (¤¤¤¤) = p < 0.05 - p < 0.0001 increase compared to NIST2975 of same dose, (‘) – (““) = p < 0.05 - p < 0.0001 increase compared to NIST1650 of same dose. Data are shown as Mean ± SEM (× 103)
  3. BAL broncho-alveloar lavage, CAP commercial airport particles, JEP jet engine particles, CB carbon black Printex 90