Fig. 2

Confocal Images of a LUHMES after 7 days of differentiation, expressing RFP or stained with neuronal markers MAP2 (green), neurofilament (red), synaptophysin (green); and b 4-week BrainSpheres expressing different neural cell type markers: β-III- tubulin/PSD95, neurofilament/synaptophysin, GFAP/O4. Scale bars 100 μm for the first three panels and 50 μm for the last panel with higher magnification