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Fig. 2 | Particle and Fibre Toxicology

Fig. 2

From: Iron oxide nanoparticles induce reversible endothelial-to-mesenchymal transition in vascular endothelial cells at acutely non-cytotoxic concentrations

Fig. 2

PSC-Fe2O3 at non-cytotoxic concentration induced EndMT in HUVECs. a Representative fluorescence confocal microphotographs and quantitative fluorescence intensity of HUVECs immunostatined with endothelial cell marker CD31 (green) or VE-cadherin (green) and mesenchymal marker α-SMA (red) or FSP (red), counterstained with DAPI for nuclei (blue). b Representative immunoblots and quantification of CD31, VE-cadherin, α-SMA, FSP of protein lysates from HUVECs treated with PSC-Fe2O3. Data are mean ± SD, n = 3, one-way ANOVA with Dunnett’s multiple comparisons test, *p < 0.05, ** p < 0.01, significant compared to control

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