Mitsui-7, heat-treated, and nitrogen-doped multi-walled carbon nanotubes elicit genotoxicity in human lung epithelial cells

Table 4 Cell Cycle Analysis

Cell Type	Exposure time (h)	Treatment	%G1	%S	%G2
BEAS-2B	24	Control	38 ± 2	23 ± 2	37 ± 3
		Arsenic	45 ± 3*	17 ± 2*	35 ± 2
		MWCNT-7	42 ± 4	33 ± 3*	25 ± 2*
		MWCNT-HT	36 ± 2	34 ± 5*^¥	27 ± 4*
		MWCNT-ND	39 ± 2	28 ± 4^¥	30 ± 3*
SAEC	24	Control	61 ± 2	29 ± 3	8 ± 1
		Arsenic	64 ± 3	25 ± 4*	9 ± 1
		MWCNT-7	76 ± 4*	10 ± 5*	10 ± 1
		MWCNT-HT	81 ± 1*^¥	5 ± 1*^¥	11 ± 1*
		MWCNT-ND	75 ± 1*^¥	14 ± 1*^¥	10 ± 1*
SAEC	72	Control	74 ± 5	15 ± 6	11 ± 1
		Arsenic	72 ± 2	7 ± 1*	18 ± 1*
		MWCNT-7	56 ± 2*^¥	31 ± 3*	13 ± 1
		MWCNT-HT	70 ± 6	21 ± 6*^¥	10 ± 1
		MWCNT-ND	68 ± 5*	21 ± 5*	11 ± 2

This table shows the mean percentage of cells in the G1, S, and G2 phase of the cell cycle measured via flow cytometry. The 24 h exposures used 24 μg/mL MWCNT doses whereas the 72 h exposure used 2.4 μg/mL MWCNT doses. BEAS-2B used 5 μM arsenic as a positive control whereas SAEC used 10 μM arsenic as a positive control. *Significantly different from control, p < 0.05 ¥Significantly different from other MWCNT materials at same dose, p < 0.05; ± SD

ISSN: 1743-8977