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Fig. 3 | Particle and Fibre Toxicology

Fig. 3

From: Inhaled gold nanoparticles cause cerebral edema and upregulate endothelial aquaporin 1 expression, involving caveolin 1 dependent repression of extracellular regulated protein kinase activity

Fig. 3

Caveolin-1 (Cav1) is a crucial factor in Au-NP-mediated AQP1 expression. a bEnd.3 cells with a silenced Cav1 (bEnd.3 Cav1-KD) was established as described in Materials and Methods. The mRNA and protein expression level of Cav1 and AQP1 was obviously down-regulated. b/c Both images and quantitative data proved Au-NP-mediated FAK and AKT phosphorylation (as well as ERK de-phosphorylation) were prevented in bEnd.3 Cav1-KD (N = 6). d Au-NP-mediated AQP1 induction and (e) water permeability was also ameliorated in bEnd.3 Cav1-KD, as compared to wild-type (N = 6). These data manifested the requirement of Cav1 of bEnd.3 cell in respond to Au-NP stimulation. (* p < 0.05, ** p < 0.01, and *** p < 0.001 indicates statistically significant difference from the control group; ## p < 0.01, and ### p < 0.001 indicates statistically significant difference from the Au-NP-treated group)

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Particle and Fibre Toxicology

ISSN: 1743-8977

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