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Fig. 5 | Particle and Fibre Toxicology

Fig. 5

From: Safer-by-design flame-sprayed silicon dioxide nanoparticles: the role of silanol content on ROS generation, surface activity and cytotoxicity

Fig. 5

Confocal images of intracellular ROS generation as a measure of CM-H2DCFDA fluorescence in RAW264.7 cells treated with the highest delivered concentration (0.104 μg/cm2) after 24 h (A) and 72 h (B). Lowercase letters correspond to the different SiO2 NPs: (a) WetChem SiO2 and FSP SiO2 (b) 5/5, (c) 3/5, (d) 4/5, (e) 9/3 and (f) 11/3, and (g) commercial fumed SiO2. Controls: (C-) negative control and (C+) positive control treated with H2O2. . Two images were obtained corresponding to green fluorescence channel (left) and transmitted light image (right)

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