Table 1 NM treatment and toxicological end-points measured in the repeated exposure experiments over a period of 3 weeks
Experiment A | End-points investigated |
Material dosing 1 at day 0 | – |
Medium replacement at day 1 24 h recovery period | AK assay, AST |
Material dosing 2 at day 2 | – |
Medium replacement at day 3 24 h recovery period | Cytokine secretion, albumin |
Material dosing 3 at day 4 | – |
Medium replacement at day 5 24 h recovery period | AK assay |
Material dosing 4 at day 6 | |
Medium replacement at day 7 | Cytokine secretion, albumin |
2 weeks recovery (medium change every other day) - collect supernatant day 21 | AK assay, cytokine secretion |
| |
Experiment B | End-points investigated |
Material dosing 1 at day 0 | – |
Medium replacement at day 1 24 h recovery period | – |
Material dosing 2 at day 2 | – |
Medium replacement at day 3 24 h recovery period | – |
Material dosing 3 at day 4 | – |
Medium replacement at day 5 24 h recovery | – |
Material dosing 4 at day 6 | – |
Medium replacement at day 7 24 h recovery | |
Material dosing 5 at day 8 | – |
Medium replacement at day 9 24 h recovery | Albumin, AST |
Material dosing 6 at day 10 | |
Medium replacement at day 11 24 h recovery | AK assay |
Material dosing 7 at day 12 | Treatment missed |
Medium replacement at day 13 24 h recovery | |
Material dosing 8 at day 14 | |
Medium replacement at day 15 | Cytokine secretion, albumin |
1 week recovery (medium change every other day) - collect supernatant day 21 | AK assay, cytokine secretion |
| |
Experiment C | End-points investigated |
Material dosing 1 at day 0 | – |
Medium replacement at day 1 24 h recovery period | – |
Material dosing 2 at day 2 | – |
Medium replacement at day 3 24 h recovery period | – |
Material dosing 3 at day 4 | – |
Medium replacement at day 5 24 h recovery | – |
Material dosing 4 at day 6 | – |
Medium replacement at day 7 24 h recovery | – |
Material dosing 5 at day 8 | – |
Medium replacement at day 9 24 h recovery | – |
Material dosing 6 at day 10 | – |
Medium replacement at day 11 24 h recovery | – |
Material dosing 7 at day 12 | Treatment missed |
Medium replacement at day 13 24 h recovery | |
Material dosing 8 at day 14 | – |
Medium replacement at day 15 24 h recovery | |
Material dosing 9 at day 16 | |
Medium replacement at day 17 24 h recovery | AST |
Material dosing 10 at day 18 | |
Medium replacement at day 19 24 h recovery | AK assay, albumin |
Material dosing 11 at day 20 | |
Collect supernatant day 21 | AK assay, cytokine secretion, AST |
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