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Table 1 NM treatment and toxicological end-points measured in the repeated exposure experiments over a period of 3 weeks

From: Assessment of nanomaterial-induced hepatotoxicity using a 3D human primary multi-cellular microtissue exposed repeatedly over 21 days - the suitability of the in vitro system as an in vivo surrogate

Experiment AEnd-points investigated
Material dosing 1 at day 0
Medium replacement at day 1      24 h recovery periodAK assay, AST
Material dosing 2 at day 2
Medium replacement at day 3      24 h recovery periodCytokine secretion, albumin
Material dosing 3 at day 4
Medium replacement at day 5      24 h recovery periodAK assay
Material dosing 4 at day 6 
Medium replacement at day 7Cytokine secretion, albumin
2 weeks recovery (medium change every other day) - collect supernatant day 21AK assay, cytokine secretion
Experiment BEnd-points investigated
Material dosing 1 at day 0
Medium replacement at day 1      24 h recovery period
Material dosing 2 at day 2
Medium replacement at day 3      24 h recovery period
Material dosing 3 at day 4
Medium replacement at day 5      24 h recovery
Material dosing 4 at day 6
Medium replacement at day 7      24 h recovery 
Material dosing 5 at day 8
Medium replacement at day 9      24 h recoveryAlbumin, AST
Material dosing 6 at day 10 
Medium replacement at day 11      24 h recoveryAK assay
Material dosing 7 at day 12Treatment missed
Medium replacement at day 13      24 h recovery 
Material dosing 8 at day 14 
Medium replacement at day 15Cytokine secretion, albumin
1 week recovery (medium change every other day) - collect supernatant day 21AK assay, cytokine secretion
Experiment CEnd-points investigated
Material dosing 1 at day 0
Medium replacement at day 1      24 h recovery period
Material dosing 2 at day 2
Medium replacement at day 3      24 h recovery period
Material dosing 3 at day 4
Medium replacement at day 5      24 h recovery
Material dosing 4 at day 6
Medium replacement at day 7      24 h recovery
Material dosing 5 at day 8
Medium replacement at day 9      24 h recovery
Material dosing 6 at day 10
Medium replacement at day 11      24 h recovery
Material dosing 7 at day 12Treatment missed
Medium replacement at day 13      24 h recovery 
Material dosing 8 at day 14
Medium replacement at day 15      24 h recovery 
Material dosing 9 at day 16 
Medium replacement at day 17      24 h recoveryAST
Material dosing 10 at day 18 
Medium replacement at day 19      24 h recoveryAK assay, albumin
Material dosing 11 at day 20 
Collect supernatant day 21AK assay, cytokine secretion, AST