Role of Nrf2 in inflammatory response in lung of mice exposed to zinc oxide nanoparticles

Table 1 Body and lung weight of mice at 14 days after exposure to zinc oxide nanoparticles by pharyngeal aspiration

		Amount of ZnO-NPs administered (μg)			Simple regression	Multiple regression
	Genotype	0	10	30	Effect of ZnO-NPs	Interaction of ZnO-NPs and Nrf2 deletion	Effect of ZnO-NPs	Effect of Nrf2 deletion
Body weight (g)	Wild type Nrf2^−/−	25.9 ± 2.0 26.7 ± 1.9	26.1 ± 1.5 26.4 ± 1.9	25.2 ± 1.6 25.9 ± 2.1	−0.027 (p = 0.29) − 0.026 (p = 0.42)	0.00057 (p = 0.97)	0.020 (p = 0.18)	0.36 (p = 0.22)
Lung weight (mg)	Wild type Nrf2^−/−	327 ± 34 331 ± 37	352 ± 36 339 ± 36	369 ± 56 378 ± 25^a	1.3 (p = 0.043) 1.6 (p = 0.0076)	0.00014(p = 0.75)	0.0014(p = 0.0011)	−8.3 × 10^− 5(p = 0.99)
Ratio of lung weight to body weight (×10⁻³)	Wild type Nrf2^−/−	12.6 ± 0.9 12.4 ± 1.1	13.5 ± 1.0 12.8 ± 1.1	14.6 ± 1.9* 14.7 ± 1.5*	0.066 (p = 0.0019) 0.077 (p = 0.0008)	−0.0057 (p = 0.68)	0.07 (p < 0.0001)	--0.14(p = 0.41)

Mean ± SD. ^* p < 0.05compared to the corresponding genotype control by Dunnett’s multiple comparison following ANOVA. Simple regression analysis in each genotype and multiple regression analysis in a model with interaction was conducted. As the interaction was not significant, multiple regression in a model without interaction was finally conducted to estimate effect of ZnO-NPs or Nrf2 deletion

ISSN: 1743-8977