From: Role of Nrf2 in inflammatory response in lung of mice exposed to zinc oxide nanoparticles
 |  | Amount of ZnO-NPs administered (μg) | Simple regression/Simple ordinal logistic regression | Multiple regression/Multiple ordinal logistic regression | ||||
---|---|---|---|---|---|---|---|---|
Genotype | 0 | 10 | 30 | Effect of ZnO-NPs | Interaction of ZnO-NPs and Nrf2 deletion | Effect of ZnO-NPs | Effect of Nrf2 deletion | |
Total cells (× 104) | Wild type Nrf2−/− | 3 ± 1.7 3.7 ± 1.5 | 3.5 ± 2.0 6.3 ± 2.5 | 9.1 ± 2.9* 13.2 ± 6.0* | 0.21(p < 0.0001) 0.32(p < 0.0001) | 0.053(p = 0.12) | 0.26(p < 0.0001) | 1.3(p = 0.0041) |
Macrophages (× 104) | Wild type Nrf2−/− | 3.1 ± 1.6 3.7 ± 1.5 | 3.5 ± 2.0 6.0 ± 2.3 | 8.4 ± 2.6* 12,0 ± 5.4* | 0.19 (p < 0.0001) 0.28 (p < 0.0001) | 0.047 (p = 0.14) | 0.23(p < 0.0001) | 1.1 (p = 0.0071) |
Lymphocytes (× 104) | Wild type Nrf2−/− | 0.04 ± 0.04 0.04 ± 0.04 | 0.10 ± 0.12 0.26 ± 0.27 | 0.58 ± 0.32* 0.9 ± 0.7* | 0.019 (p < 0.0001) 0.029 (p = 0.0002) | 0.0051 (p = 0.15) | 0.024(p < 0.0001) | 0.081(p = 0.074) |
Neutrophils (× 104) | Wild type Nrf2−/− | 0 0 | 0.01 ± 0.01 0.003 ± 0.004 | 0.02 ± 0.02 0.074 ± 0.07* | 0.066 (p = 0.059) 0.16 (p = 0.002) | 0.11 (p = 0.06) | 0.11 (p = 0.0002) | 0.70 (p = 0.28) |
Eosinophils (× 104) | Wild type Nrf2−/− | 0 0 | 0.003 ± 0.005 0.013 ± 0.013* | 0.04 ± 0.04* 0.16 ± 0.3* | 0.16 (p = 0.0006) 0.24 (p = 0.0004) | 0.016 (p = 0.76) | 0.18 (p < 0.0001) | 1.3 (p = 0.046) |
Total protein (mg/ml) | Wild type Nrf2−/− | 0.08 ± 0.02 0.08 ± 0.02 | 0.09 ± 0.03 0.08 ± 0.02 | 0.08 ± 0.02 0.07 ± 0.02 | −0.00014 (p = 0.72) − 0.00028 (p = 0.35) | 6.7 × 10−5 (p = 0.79) | 0.00025 (p = 0.43) | −0.002(p = 0.67) |