Role of Nrf2 in inflammatory response in lung of mice exposed to zinc oxide nanoparticles

Table 3 Total inflammation score, perivascular inflammation score and peribronchial inflammation score from the mice at 14 days after exposure to zinc oxide nanoparticles by pharyngeal aspiration

		Amount of ZnO-NPs administered (μg)			Simple ordinal logistic regression	Multiple ordinal logistic regression
	Genotype	0	10	30	Effect of ZnO-NPs	Interaction of ZnO-NPs and Nrf2 deletion	Effect of ZnO-NPs	Effect of Nrf2 deletion
Total inflammation score	Wild type Nrf2^−/−	0 ± 0 0.13 ± 0.35	1.5 ± 0.9* 1.6 ± 0.9*	2.8 ± 0.5* 2.7 ± 0.5*	0.23(p < 0.0001) 0.20(p = 0.0002)	−0.060 (p = 0.27)	0.21(p < 0.0001)	0.078 (p = 0.89)
Perivascular inflammation	Wild type Nrf2^−/−	0 ± 0 0.13 ± 0.35	1.4 ± 1.0* 1.4 ± 0.7*	2.7 ± 0.7* 2.6 ± 0.5*	0.23 (p < 0.0001) 0.41 (p = 0.0056)	−0.055 (p = 0.32)	0.24(p < 0.0001)	−0.033 (p = 0.96)
Peribronchial inflammation	Wild type Nrf2^−/−	0 ± 0 0.13 ± 0.35	1.6 ± 1.0* 1.9 ± 1.1*	2.8 ± 0.4* 2.8 ± 0.5*	0.23(p < 0.0001) 0.17 (p = 0.0005)	−0.035 (p = 0.56)	0.20 (p < 0.0001)	0.33 (p = 0.59)

Mean ± SD. ^* p < 0.05, compared to the corresponding genotype control by Steel multiple comparison following Kruskal Wallis nonparametric test. Simple ordinal logistic regression analysis in each genotype and multiple ordinal logistic regression analysis in a model with interaction was conducted. As the interaction was not significant, multiple ordinal logistic regression analysis was finally conducted in a model without interaction to estimate effect of ZnO-NPs or Nrf2 deletion

ISSN: 1743-8977