Table 4 Total GSH, GSSG, ratio of GSSG/total GSH and MDA in the lung of mice at 14 days after exposure to zinc oxide nanoparticles by pharyngeal aspiration
From: Role of Nrf2 in inflammatory response in lung of mice exposed to zinc oxide nanoparticles
| Amount of ZnO-NPs administered (μg) | Simple regression | Multiple regression | ||||||
|---|---|---|---|---|---|---|---|---|
| Genotype | 0 | 10 | 30 | Effect of ZnO-NPs | Interaction of ZnO-NPs and Nrf2 deletion | Effect of ZnO-NPs | Effect of Nrf2 deletion | |
| Total glutathione (GSH + GSSG, nmol / g lung tissue) | Wild type Nrf2−/− | 47.9 ± 6.5 42.9 ± 10.4 | 41.4 ± 7.3 44.9 ± 7.5 | 45.4 ± 7.8 51.5 ± 5.2 | −0.041 (p = 0.71) 0.29 (p = 0.032) | 0.17 (p = 0.053) | 0.11 (p = 0.22) | 0.78 (p = 0.48) |
| Glutathione disulfide (GSSG, nmol / g lung tissue) | Wild type Nrf2−/− | 5.8 ± 2.6 8.0 ± 2.2 | 5.1 ± 1.1 8.2 ± 2.0 | 6.2 ± 2.2 4.9 ± 2.0* | 0.020 (p = 0.52) − 0.11 (p = 0.0036) | − 0.066 (p = 0.0057) | – | – |
| Ratio of GSSG/GSH (×10−1) | Wild type Nrf2−/− | 1.48 ± 0.37 2.37 ± 0.67 | 1.45 ± 0.30 2.22 ± 4.41 | 1.62 ± 0.67 1.08 ± 0.54* | 0.00052(p = 0.47) −0.0045 (p < 0.0001) | −0.0025 (p < 0.0001) | – | – |
| MDA (nmol / mg protein) | Wild type Nrf2−/− | 2.34 ± 0.37 1.42 ± 0.18 | 2.27 ± 0.27 1.38 ± 0.47 | 1.27 ± 0.19* 1.97 ± 0.67 | −0.038 (p < 0.0001) 0.020 (p = 0.019) | 0.029 (p < 0.0001) | – | – |