Table 5 Relative mRNA level for oxidative stress-related proteins to β-actin
From: Role of Nrf2 in inflammatory response in lung of mice exposed to zinc oxide nanoparticles
| Amount of ZnO-NPs administered (μg) | Simple regression | Multiple regression | ||||||
|---|---|---|---|---|---|---|---|---|
| Genotype | 0 | 10 | 30 | Effect of ZnO-NPs | Interaction of ZnO-NPs and Nrf2 deletion | Effect of ZnO-NPs | Effect of Nrf2 deletion | |
| SOD1 | Wild type Nrf2−/− | 1.5 ± 0.4 1.02 ± 0.3 | 1.8 ± 0.7 1.5 ± 0.3* | 2.3 ± 0.9 1.2 ± 0.3 | 0.025(p = 0.027) 0.0023 (p = 0.68) | −0.011 (p = 0.083) | 0.014(p = 0.028) | −0.31(p = 0.0003) |
| CAT | Wild type Nrf2−/− | 2.4 ± 0.5 2.6 ± 0.7 | 1.9 ± 0.7 2.7 ± 1.0 | 3.2 ± 1.5(p = 0.042) 2.3 ± 0.8 | 0.030 (p = 0.063) −0.011 (p = 0.45) | −0.02 (p = 0.06) | 0.012 (p = 0.28) | 0.011 (p = 0.94) |
| GcLc | Wild type Nrf2−/− | 1.6 ± 0.5 1.3 ± 0.4 | 1.4 ± 0.4 1.6 ± 0.5 | 1.9 ± 0.9 1.1 ± 0.2 | 0.011(p = 0.25) −0.011 (p = 0.14) | −0.011 (p = 0.082) | 0.0016(p = 0.80) | −0.17(p = 0.032) |
| GcLm | Wild type Nrf2−/− | 1.5 ± 0.6 1.3 ± 0.3 | 1.1 ± 0.3 1.3 ± 0.2 | 1.3 ± 0.5 1.0 ± 0.3 | −0.0033 (p = 0.65) − 0.0095 (p = 0.48) | −0.0031 (p = 0.50) | − 0.0061 (p = 0.18) | −0.057(p = 0.32) |
| NQO1 | Wild type Nrf2−/− | 0.9 ± 0.5 0.7 ± 0.1 | 0.9 ± 0.8 0.9 ± 0.3 | 0.9 ± 0.8 0.6 ± 0.1 | −0.00095 (p = 0.93) − 0.0045 (p = 0.23) | −0.0018 (p = 0.77) | − 0.0025 (p = 0.67) | −0.064(p = 0.39) |
| GR | Wild type Nrf2−/− | 1.3 ± 0.7 2.1 ± 0.5 | 0.9 ± 0.3 1.8 ± 0.5 | 4.6 ± 6.7 1.7 ± 0.7 | 0.12(p = 0.040) −0.011 (p = 0.24) | −0.067 (p = 0.043) | – | – |
| HO-1 | Wild type Nrf2−/− | 7.5 ± 3.0 5.8 ± 2.5 | 7.1 ± 2.3 3.8 ± 1.6 | 6.8 ± 1.9 4.2 ± 1.7 | −0.023 (p = 0.53) − 0.042 (p = 0.22) | 0.0095 (p = 0.70) | − 0.031 (p = 0.21) | −1.25(p = 0.0002) |
| MT-1 | Wild type Nrf2−/− | 1.5 ± 0.9 1.9 ± 1.1 | 1.4 ± 0.6 2.2 ± 0.6 | 3.8 ± 2.5* 2.6 ± 0.9 | 0.081(p = 0.0017) −0.0033 (p = 0.83) | −0.042 (p = 0.0063) | – | – |
| MT-2 | Wild type Nrf2−/− | 1.9 ± 0.7 3.7 ± 1.6 | 1.9 ± 0.8 3.8 ± 1.4 | 3.9 ± 2.0* 4.1 ± 2.5 | 0.072 (p = 0.001) 0.013(p = 0.66) | −0.029 (p = 0.097) | 0.046 (p = 0.012) | 0.68(p = 0.0035) |