Table 6 Relative mRNA level for pro-inflammatory cytokines and proteins related with fibrosis to β-actin
From: Role of Nrf2 in inflammatory response in lung of mice exposed to zinc oxide nanoparticles
| Amount of ZnO-NPs administered (μg) | Simple regression | Multiple regression | ||||||
|---|---|---|---|---|---|---|---|---|
| Genotype | 0 | 10 | 30 | Effect of ZnO-NPs | Interaction of ZnO-NPs and Nrf2 deletion | Effect of ZnO-NPs | Effect of Nrf2 deletion | |
| KC | Wild type Nrf2−/− | 1.0 ± 0.4 2.2 ± 1.3 | 1.4 ± 0.7 2.8 ± 1.0 | 1.2 ± 0.4 2.9 ± 1.4 | 0.0026 (p = 0.73) 0.023(p = 0.27) | 0.010 (p = 0.30) | 0.012(p = 0.25) | −0.71 (p < 0.0001) |
| MIP-2 | Wild type Nrf2−/− | 1.1 ± 0.6 2.1 ± 1.1 | 1.3 ± 0.8 2.4 ± 1.2 | 1.0 ± 0.3 3.9 ± 3.0 | −0.0060 (p = 0.51) 0.064(p = 0.073) | 0.035 (p = 0.028) | – | – |
| IL-6 | Wild type Nrf2−/− | 1.1 ± 0.4 1.1 ± 0.4 | 0.9 ± 0.3 1.1 ± 0.3 | 1.2 ± 0.6 1.5 ± 0.8 | 0.005(p = 0.48) 0.015(p = 0.14) | 0.0049 (p = 0.39) | 0.0090 (p = 0.11) | 0.078 (p = 0.28) |
| IL-1β | Wild type Nrf2−/− | 1.0 ± 0.7 0.9 ± 0.4 | 1.0 ± 0.6 1.1 ± 0.3 | 0.7 ± 0.3 1.1 ± 0.7 | −0.0097 (p = 0.24) 0.005(p = 0.58) | 0.0073 (p = 0.23) | −0.0034 (p = 0.58) | 0.032 (p = 0.68) |
| MCP-1 | Wild type Nrf2−/− | 0.9 ± 0.7 0.7 ± 0.3 | 0.9 ± 1.0 0.6 ± 0.3 | 0.7 ± 0.4 0.9 ± 0.6 | −0.0088 (p = 0.41) 0.0055 (p = 0.48) | 0.0072 (p = 0.31) | −0.0027 (p = 0.70) | −0.038 (p = 0.67) |
| TGF-β | Wild type Nrf2−/− | 1.1 ± 0.3 1.3 ± 0.2 | 1.1 ± 0.2 1.5 ± 0.7 | 1.6 ± 0.5* 1.4 ± 0.3 | 0.019 (p = 0.0011) 0.0028 (p = 0.70) | --0.0083 (p = 0.066) | 0.012(p = 0.0098) | 0.084 (p = 0.14) |
| TNF-α | Wild type Nrf2−/− | 2.9 ± 1.0 2.9 ± 0.8 | 2.8 ± 0.6 2.6 ± 1.2 | 2.5 ± 0.7 3.1 ± 0.9 | −0.012 (p = 0.30) 0.0089(p = 0.58) | 0.010 (p = 0.28) | −0.0026 (p = 0.79) | 0.092 (p = 0.43) |
| IFN-γ | Wild type Nrf2−/− | 1.5 ± 0.6 3.5 ± 2.2 | 1.6 ± 0.5 4.0 ± 3.2 | 2.7 ± 0.9* 4.5 ± 3.9 | 0.042(p = 0.0004) 0.031 (p = 0.55) | --0.0053 (p = 0.82) | 0.037 (p = 0.11) | 1.1 (p = 0.0006) |
| MMP2 | Wild type Nrf2−/− | 0.7 ± 0.2 1.1 ± 0.4 | 0.7 ± 0.2 1.2 ± 0.4 | 1.5 ± 0.4* 1.9 ± 0.7* | 0.027 (p = < 0.0001) 0.029 (p = 0.0022) | 0.00092 (p = 0.84) | 0.028 (p < 0.0001) | 0.22 (p = 0.0002) |