Particle and Fibre Toxicology

Table 2 Size characterization of SAS in stock suspensions and exposure media (for HBE, Caco2 and THP-1) using DLS

From: Is aggregated synthetic amorphous silica toxicologically relevant?

		Stock		HBE		Caco2		THP1
AM		Z-avg	PDI	Z-avg	PDI	Z-avg	PDI	Z-avg	PDI
DE-AGGR	0 h	264	0.33	228	0.35	225	0.34	228	0.36
DE-AGGR	24 h	260	0.32	233	0.37	1275	0.28	226	0.42
AGGR	0 h	10,040	0.90	3212	0.72	4321	0.82	3566	0.97
AGGR	24 h	12,374	0.88	3059	0.80	1637	0.46	2688	0.75
SuperN	0 h	3953	0.94	4507	0.87	4500	0.89	4507	0.95
SuperN	24 h	3661	1.00	3851	0.83	2233	0.85	3866	0.95
PREC	0 h	3332	0.90	1101	0.85	1370	0.83	1561	0.93
PREC	24 h	4329	0.96	957	0.88	1376	0.82	955	0.91

Freshly prepared stock suspensions were diluted to 100 μg/mL in different exposure media (without serum) and, hydrodynamic sizes (Z-avg) and polydispersity index (PDI) were measured directly and after 24 h (incubated at 37 °C)

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ISSN: 1743-8977

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