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Fig. 7 | Particle and Fibre Toxicology

Fig. 7

From: Silica nanomaterials induce organ injuries by Ca2+-ROS-initiated disruption of the endothelial barrier and triggering intravascular coagulation

Fig. 7

Confocal images exhibiting the effects of SiNPs on the junctional integrity of VE-cadherin (green) and assembly of F-actin (red) in HUVEC cultures. Nuclei were stained blue by DAPI. HUVECs monolayer showed normal distribution of VE-cadherin and F-actin before exposure to SiNPs (upper row). After exposure to SiNP-20 (100 μg/mL), HUVECs monolayer showed larger cell size, discontinued junctional pattern of VE-cadherin (red arrow) and re-assembly of F-actin (white arrow) which suggests F-actin remodeling (middle row). SiNP-100 exerted similar effects on cell size, VE-cadherin continuity (red arrow) and F-actin re-assembly (white arrow), and was likely stronger than SiNP-20 in inducing F-actin remodeling (lower row). Right column was the merged images of VE-cadherin and F-actin. Scale bar = 10 μm

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