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Fig. 9 | Particle and Fibre Toxicology

Fig. 9

From: Silica nanomaterials induce organ injuries by Ca2+-ROS-initiated disruption of the endothelial barrier and triggering intravascular coagulation

Fig. 9

Morphological manifestation of the toxic effects of SiNPs (35 mg/kg, i.v.) on the heart, aorta and lung in mice in vivo. a, normal control myocardium (H&E stain). b, SiNP-20 induced adherence of red blood cells to the luminal side of a coronary artery (black arrow). c, SiNP-100 induced intracoronary coagulation (black arrows), clog shrinkage was seen inside the coronary artery (inset). d, SiNP-100 induced cell-containing neoplasm formation in the luminal side of an abdominal aorta (black arrows in the inset which was an enlargement of the framed area). e-j, immunohistochemical stains of F4/80 (a macrophage marker) in the myocardium (e, f, g) and abdominal aorta (h, i, j) of mice in vivo. Note that F4/80 was negative in control myocardium and aortic wall, but was positive (brown) in mice exposed to SiNP-20 or SiNP-100 for 72 h, suggesting macrophage infiltration and inflammation in these tissues. k, l and m, H&E stains of control lung tissue (k) and lung tissues from mice exposed to SiNP-20 (l) or SiNP-100 (m) for 72 h. Both SiNP-20 and SiNP-100 induced pulmonary vein coagulation (black arrows in panels l and m) and bronchiolic lumen oozing, coagulation and narrowing (blue arrows in panels l and m). n, o and p, immunohistochemical stains of F4/80 in lung tissues, indicating that SiNP-20 and SiNP-100 induced macrophage infiltration in the lung. Scale bar = 100 μm

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