Quantitative biokinetics over a 28 day period of freshly generated, pristine, 20 nm silver nanoparticle aerosols in healthy adult rats after a single 1½-hour inhalation exposure

Table 1 Design of the [¹⁰⁵Ag]AgNP biokinetics study and the auxiliary studies after intravenous injection, intratracheal instillation, and intra-oral instillation (gavage) of [^110mAg]AgNO₃

Female, healthy, adult WKY rats, 8–10 weeks old		dissection time-points for biodistribution analyses
		0.75 h/1st^a	0.75 h/2nd^b	4 h	24 h/1st	24 h/2nd^b	7d	28d
[¹⁰⁵Ag]AgNP Inhalation, 1½ -hour exposure	# of rats	4	4	4	4	4	4	4
Intratravenous injection of soluble [^110mAg]AgNO₃	# of rats				4		4
Intratracheal instillation of soluble [^110mAg]AgNO₃	# of rats				4		4
Oral instillation (gavage) of soluble [^110mAg]AgNO₃	# of rats				4		4

^a Note: rats were exsanguinated and dissected immediately after the 1.5-h exposure; “0.75 h” represents the mean time-point of the 1.5-h exposure
^b The measurements at 0.75 h and 24 h p.e. were repeated using a second group of rats, respectively, in order to test data reproducibility during the rapidly changing biokinetics directly after inhalation
Study design of the [¹⁰⁵Ag]AgNP biokinetics study in adult rats from immediately after inhalation up to 28 days p.e. The number of rats at each dissection time-point is given. Auxiliary biokinetics studies were performed after intratracheal instillation, intravenous injection, and oral instillation (gavage) of [^110mAg]AgNO₃ solutions at two dissection time points 24 h and 7 days p.e.

ISSN: 1743-8977