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Table 4 [105Ag]AgNP retention in lungs and BAL, and in secondary organs and tissues over time p.e

From: Quantitative biokinetics over a 28 day period of freshly generated, pristine, 20 nm silver nanoparticle aerosols in healthy adult rats after a single 1½-hour inhalation exposure

dissection time

 

0.75 h(1st)

0.75 h(2nd)

4 h

24 h(1st)

24 h(2nd)

7d

28d

organ

 

mean ± STD

mean ± STD

mean ± STD

mean ± STD

mean ± STD

mean ± STD

mean ± STD

lungs + BAL

Corr. resid. Blood

0.9 ± 0.02

0.88 ± 0.05

0.68 ± 0.01

0.55 ± 0.03

0.62 ± 0.05

0.14 ± 0.09

0.06 ± 0.031

lungs + BAL

fract. Conc. [1/g]

0.72 ± 0.02

0.7 ± 0.04

0.75 ± 0.04

0.67 ± 0.02

0.64 ± 0.05

0.23 ± 0.06

0.11 ± 0.06

lungs+BAL(*103)

mass conc. [ng/g]

16.08 ± 5.1

14.48 ± 4.59

15.17 ± 3.41

12.23 ± 2.29

12.16 ± 4.29

2.92 ± 3.12

1.16 ± 0.46

lav. Lungs

Corr. resid. Blood

0.64 ± 0.06

0.65 ± 0.1

0.51 ± 0.03

0.47 ± 0.02

0.54 ± 0.05

0.13 ± 0.09

0.06 ± 0.03

lav. Lungs

fract. Conc. [1/g]

0.56 ± 0.05

0.57 ± 0.07

0.61 ± 0.04

0.66 ± 0.03

0.61 ± 0.06

0.24 ± 0.07

0.12 ± 0.07

lav. Lungs (*103)

mass conc. [μg/g]

12.71 ± 4.69

11.87 ± 4.5

12.43 ± 3

11.95 ± 1.84

11.5 ± 4.32

3.03 ± 3.28

1.25 ± 0.51

BALC(*10− 2)

Corr. resid. Blood

3.86 ± 0.67

3.74 ± 0.73

10.2 ± 1.09

4.18 ± 1.25

4.92 ± 0.76

0.33 ± 0.14

0.03 ± 0.02

BALC

fract. Conc. [1/g]

3.94 ± 0.69

3.97 ± 0.97

13.97 ± 1.38

6.48 ± 1.8

7.02 ± 1.27

0.82 ± 0.21

0.06 ± 0.04

BALC(*104)

mass conc. [ng/g]

8.51 ± 1.6

7.98 ± 2.28

28.21 ± 6.02

11.97 ± 4.32

12.62 ± 1.04

0.9 ± 0.76

0.09 ± 0.07

BALF(*10− 1)

Corr. resid. Blood

2.22 ± 0.47

1.89 ± 0.45

0.72 ± 0.12

0.33 ± 0.09

0.34 ± 0.03

0.03 ± 0.012

0.001 ± 0

BALF

fract. Conc. [1/g]

2.27 ± 0.48

2.01 ± 0.57

0.98 ± 0.16

0.51 ± 0.14

0.48 ± 0.03

0.076 ± 0.025

0.002 ± 0.001

BALF(*104)

mass conc. [ng/g]

4.89 ± 0.98

3.93 ± 0.66

1.96 ± 0.39

0.93 ± 0.32

0.91 ± 0.31

0.079 ± 0.058

0.002 ± 0.001

tra. + bron.(*10− 3)

Corr. resid. Blood

    

10 ± 3.1

2.93 ± 1.6

1.86 ± 1.99

tra. + bron.(*10− 1)

fract. Conc. [1/g]

    

0 ± 0

0.7 ± 0.45

0.46 ± 0.73

tra. + bron.(*104)

mass conc. [ng/g]

    

0 ± 0

0.05 ± 0.03

0.03 ± 0.04

liver(*10− 3)

Corr. resid. Blood

5.31 ± 2.16

5.02 ± 1.82

12.05 ± 1.27

19.54 ± 1.95

17.37 ± 3.72

2.66 ± 0.61

0.5 ± 0.14

liver(*10− 3)

fract. Conc. [1/g]

0.7 ± 0.3

0.72 ± 0.3

2.39 ± 0.27

4.26 ± 0.8

3.33 ± 0.34

0.84 ± 0.14

0.16 ± 0.05

liver(*101)

mass conc. [ng/g]

1.49 ± 0.58

1.45 ± 0.67

4.78 ± 0.87

7.6 ± 0.54

6.13 ± 1.41

0.84 ± 0.48

0.18 ± 0.08

liver(*100)

transloc. NP fract.

0.21 ± 0.04

0.22 ± 0.11

0.3 ± 0.05

0.24 ± 0.03

0.26 ± 0.08

0.11 ± 0.01

0.03 ± 0.01

spleen(*10− 4)

Corr. resid. Blood

0.78 ± 0.54

3.52 ± 4

1 ± 0.33

1.69 ± 0.1

1.52 ± 0.06

0.76 ± 0.2

0.28 ± 0.13

spleen(*10− 4)

fract. Conc. [1/g]

1.69 ± 1.71

10.76 ± 12.52

3.72 ± 1.21

7.92 ± 1.12

3.77 ± 2.25

4.26 ± 0.54

1.11 ± 0.47

spleen(*100)

mass conc. [ng/g]

4.79 ± 3.44

25.6 ± 36.22

7.3 ± 2.16

14.2 ± 0.99

7.6 ± 5.43

4.34 ± 2.65

1.41 ± 0.81

spleen(*10− 2)

transloc. NP fract.

0.29 ± 0.13

0.86 ± 0.82

0.25 ± 0.08

0.21 ± 0.01

0.23 ± 0.08

0.3 ± 0.02

0.15 ± 0.11

kidneys(*10− 3)

Corr. resid. Blood

0.39 ± 0.12

0.41 ± 0.22

1.24 ± 0.09

5.16 ± 0.94

6.66 ± 1.35

1.31 ± 0.78

0.11 ± 0.04

kidneys(*10−3)

fract. Conc. [1/g]

0.23 ± 0.09

0.24 ± 0.13

0.95 ± 0.13

4.6 ± 0.76

9.33 ± 8.15

1.47 ± 0.5

0.19 ± 0.09

kidneys(*100)

mass conc. [ng/g]

4.71 ± 0.72

5 ± 3.51

18.81 ± 1.95

83.97 ± 19.3

152.7 ± 98.83

17.16 ± 15.66

2.03 ± 0.39

kidneys(*10− 2)

transloc. NP fract.

1.58 ± 0.38

1.55 ± 0.3

3.12 ± 0.71

6.29 ± 1.15

10.04 ± 3.67

4.81 ± 1.27

0.55 ± 0.16

heart (*10− 4)

Corr. resid. Blood

0.95 ± 0.18

7.07 ± 11.36

2.48 ± 0.52

4.14 ± 0.35

5.07 ± 0.62

0.9 ± 0.53

0.05 ± 0.01

heart (*10−4)

fract. Conc. [1/g]

1.32 ± 0.3

9.22 ± 14.74

4.85 ± 1.02

9.43 ± 0.91

9.59 ± 1.8

2.93 ± 1.88

0.16 ± 0.04

heart (*100)

mass conc. [ng/g]

2.98 ± 1.25

25.2 ± 44.1

9.53 ± 0.85

17.03 ± 1.83

17.87 ± 6.3

2.62 ± 1.33

0.21 ± 0.12

heart (*10− 2)

transloc. NP fract.

0.41 ± 0.15

1.91 ± 2.45

0.63 ± 0.2

0.5 ± 0.04

0.76 ± 0.24

0.39 ± 0.29

0.03 ± 0.01

brain(*10−4)

Corr. resid. Blood

0.41 ± 0.56

0.46 ± 0.37

0.26 ± 0.08

1.62 ± 0.22

1.09 ± 0.07

3.43 ± 0.74

3.64 ± 0.32

brain(*10− 4)

fract. Conc. [1/g]

0.22 ± 0.29

0.28 ± 0.23

0.2 ± 0.06

1.39 ± 0.2

0.89 ± 0.1

6.64 ± 4.7

4.65 ± 0.3

brain(*100)

mass conc. [ng/g]

0.37 ± 0.35

0.55 ± 0.46

0.41 ± 0.16

2.5 ± 0.19

1.63 ± 0.35

5.86 ± 3.21

5.68 ± 2.41

brain(*10− 2)

transloc. NP fract.

0.17 ± 0.22

0.34 ± 0.36

0.07 ± 0.03

0.2 ± 0.03

0.16 ± 0.05

1.42 ± 0.43

1.88 ± 0.44

uterus(*10− 4)

Corr. resid. Blood

0.77 ± 0.86

3.79 ± 4.33

0.66 ± 0.67

0.84 ± 0.99

1.43 ± 2.01

0.81 ± 0.62

0.07 ± 0.02

uterus(*10−4)

fract. Conc. [1/g]

1.45 ± 1.62

7.54 ± 8.53

1.79 ± 1.85

2.5 ± 2.96

3.59 ± 5.04

3.27 ± 2.1

0.29 ± 0.11

uterus(*100)

mass conc. [ng/g]

3.53 ± 3.94

17.2 ± 23.1

4.07 ± 4.3

4.87 ± 5.64

5.55 ± 6.88

4.07 ± 4.21

0.32 ± 0.12

uterus(*10−2)

transloc. NP fract.

0.3 ± 0.34

0.89 ± 0.93

0.17 ± 0.18

0.1 ± 0.12

0.17 ± 0.2

0.3 ± 0.19

0.04 ± 0.02

carcass(*10− 2)

Corr. resid. Blood

18.35 ± 4.26

21.2 ± 16.0

26.22 ± 8.23

51.01 ± 4.7

41.58 ± 24.65

13.23 ± 2.73

2.78 ± 0.14

carcass(*10− 2)

fract. Conc. [1/g]

0.12 ± 0.03

0.14 ± 0.11

0.23 ± 0.07

0.53 ± 0.05

0.33 ± 0.18

0.2 ± 0.03

0.04 ± 0

carcass(*101)

mass conc. [ng/g]

0.27 ± 0.07

0.3 ± 0.26

0.47 ± 0.18

0.97 ± 0.18

0.56 ± 0.17

0.2 ± 0.12

0.04 ± 0.02

carcass(*100)

transloc. NP fract.

0.74 ± 0.03

0.69 ± 0.1

0.63 ± 0.07

0.62 ± 0.04

0.55 ± 0.15

0.53 ± 0.06

0.14 ± 0.04

blood(*10− 3)

Corr. resid. Blood

1.55 ± 0.21

1.85 ± 1.34

4.59 ± 1.02

16.57 ± 1.64

12.47 ± 0.82

3.11 ± 0.57

0.24 ± 0.09

blood(*10− 3)

fract. Conc. [1/g]

0.12 ± 0.02

0.15 ± 0.12

0.5 ± 0.1

2.13 ± 0.33

1.3 ± 0.05

0.58 ± 0.08

0.04 ± 0.01

blood(*10− 3)

mass conc. [ng/g]

0.27 ± 0.07

0.28 ± 0.15

0.99 ± 0.14

3.8 ± 0.07

2.4 ± 0.57

0.58 ± 0.34

0.05 ± 0.03

blood(*100)

transloc. NP fract.

0.06 ± 0.02

0.07 ± 0.03

0.11 ± 0.03

0.2 ± 0.02

0.19 ± 0.06

0.13 ± 0.02

0.01 ± 0

transloc.(* 10− 2)

Corr. resid. Blood

2.51 ± 0.64

2.91 ± 1.96

4.12 ± 0.86

8.2 ± 0.24

7.2 ± 2.47

2.56 ± 0.85

2.01 ± 0.43

transloc.(* 10− 3)

fract. Conc. [1/g]

0.15 ± 0.04

0.18 ± 0.13

0.32 ± 0.07

0.76 ± 0.04

0.52 ± 0.16

0.33 ± 0.02

0.23 ± 0.05

transloc.(* 101)

mass conc. [ng/g]

0.33 ± 0.09

0.37 ± 0.31

0.66 ± 0.19

1.38 ± 0.18

0.91 ± 0.06

0.36 ± 0.269

0.29 ± 0.136

2nd org. (*10− 2)

Corr. resid. Blood

0.6 ± 0.23

0.69 ± 0.35

1.37 ± 0.14

2.55 ± 0.2

2.49 ± 0.49

0.46 ± 0.14

0.1 ± 0.02

2nd org. (*10− 3)

fract. Conc. [1/g]

0.37 ± 0.2

0.45 ± 0.25

1.14 ± 0.12

1.47 ± 0.24

1.59 ± 0.23

0.37 ± 0.12

0.15 ± 0.04

2nd org. (*101)

mass conc. [ng/g]

0.78 ± 0.38

0.98 ± 0.82

2.29 ± 0.49

2.66 ± 0.47

2.92 ± 0.66

0.4 ± 0.31

0.17 ± 0.07

2nd org. (*100)

transloc. NP fract.

0.23 ± 0.04

0.27 ± 0.09

0.34 ± 0.06

0.31 ± 0.03

0.37 ± 0.12

0.18 ± 0.01

0.05 ± 0.02

  1. [105Ag]AgNP retention in lungs including broncho-alveolar-lavage (BAL) data and in secondary organs and tissues at 0.75 h, 4 h, 24 h, 7d and 28d after intratracheal inhalation; note, for 0.75 and 24 h p.e. two groups of rats were dissected for data repeatability and statistical control. The data are presented as retained [105Ag]Ag - activity fractions normalized to the ILD of [105Ag]AgNP and corrected for 105Ag in the residual blood in each organ (first line of a given organ). In the following two lines for each organ, the [105Ag]Ag -activity data were converted into [105Ag]AgNP concentrations per mass of organ or tissue, given as ILD fraction per gram and in NP mass ng·g− 1. In the fourth line of each secondary organ or tissue, fractions are normalized to the total of the [105Ag]Ag activity, which had crossed the ABB (transloc. NP fract., see Eqns. 23 and 24 of Supplementary Information). Mean ± SEM of n = 4 rats/time point.
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Particle and Fibre Toxicology

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