Fig. 3
Effect of an ROS scavenger on the electrophysiological stability of cardiomyocytes. a Electrophysiological parameter data averages from cardiomyocytes with ambient PM infusion with or without NAC (n = 4). b Representative fluorescence images of ROS generation or intracellular calcium in PM-treated cardiomyocytes with or without NAC using H2DCF-DA (top, green) or Fluo-4 AM (under, green), respectively (n = 4). Nuclei were stained with DAPI (blue). The rate of fluorescence for every sample was quantified by SIBIA software. c The mRNA levels of CaMKII, RyR2, and SERCA2a in the absence or presence of NAC were quantified by qPCR (n = 4). Gene expression levels were normalized to GAPDH. d The expression levels of phospho-CaMKII, phospho-RyR2, and SERCA2a in the absence or presence of NAC were analyzed by western blotting (n = 5). Protein levels were normalized to β-actin. Values are represented as mean ± SD. *P < 0.05 or **P < 0.01 compared with non-treated controls