Table 3 Sequences of primers used for real-time quantitative PCR
Gene | Primer sequence |
|---|---|
GAPDH | Sense: 5′-CAGTGCCAGCCTCGTCTCAT-3′ Antisense: 5′-TGGTAACCAGGCGTCCGATA-3’ |
SERCA2a | Sense: 5’-CGAGTTGAACCTTCCCACAA-3′ Antisense: 5′-AGGAGATGAGGTAGCCGATGAA-3’ |
RyR2 | Sense: 5’-CAAACAGGGCAGAAGACACC-3′ Antisense: 5′-CTCTGAGGGTGCTCCACCT-3’ |
CaMKII | Sense: 5’-CATCCTGAACCCTCACATCCA-3′ Antisense: 5′-CCGCATCCAGGTACTGAGAGTGAT-3’ |
Calsequestrin2 | Sense: 5’-TCAAAGACCCACCCTACGTC-3′ Antisense: 5′-AGTCGTCTGGGTCAATCCAC-3’ |
CalcineurinA | Sense: 5’-TGGTGAAAGCCGTTCCATTT-3′ Antisense: 5’ CCCATCGTTATCAAACACTTCCT-3’ |
Calmodulin | Sense: 5′-GGCATCCTGCTTTAGCCTGAG-3′ Antisense: 5′-ACATGCTATCCCTCTCGTGTGAC-3’ |
NCX1 | Sense: 5’-AGCAAGGCGGCTTCTCTTTT-3′ Antisense: 5′-GCTGGTCTGTCTCCTTCATGT-3’ |
NCX2 | Sense: 5’-CACTACGAGGATGCTTGTGG-3′ Antisense: 5′-CCTTCTTCTCATACTCTTCGT-3’ |
NCX3 | Sense: 5’-CCTGTGGCTCCTCTACGTACTCTT-3′ Antisense: 5′-GAGGTCTTGTTCTGGTGGTTCA-3’ |