Fig. 7
From: Urban air particulate matter induces mitochondrial dysfunction in human olfactory mucosal cells
NPTX1-knockdown did not ameliorate mitochondrial function via ROS signalling. a Experimental timeline. b Validation of NPTX1 expression shows a significant increase in hOM cells exposed to PM2.5–1 and significant suppression of NPTX1 following RNAi in a representative cell line. NPTX1 transcripts levels are presented as log fold change (logFC) and normalized by the reference gene, GAPDH and to the scrambled control (NC1). c MTT assay results of 24 h exposure to PM after 48 h treatment with siRNA. d LDH release assay results of 24 h exposure to PM after 48 h treatment with siRNA. e Proportion of mitoSOX-positive cells increased in NPTX1-kd cells exposed to PM. f Measurement of OCR with SeahorseFX technology revealed that suppression of NPTX1 (in green lines) did not further increase the failure of cellular respiratory activity after PM exposure. g Intracellular ATP levels were not altered by NPTX1-kd. (H) STRING interaction network of NPTX1 in human. *p < 0.05 **p < 0.01 ***p < 0.0001. N = 3–6 wells/group