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Fig. 4 | Particle and Fibre Toxicology

Fig. 4

From: Silica nanoparticles induce lung inflammation in mice via ROS/PARP/TRPM2 signaling-mediated lysosome impairment and autophagy dysfunction

Fig. 4

Contribution of surface silanol groups and NADPH oxidases in SiNPs-induced oxidative stress. a Hemolysis to mouse RBCs after exposure to 25–100 μg/mL SiNPs (100 μg/mL) or calcined-SiNPs for 3 h at room temperature. The insert represents hemoglobin release indicated by red color in the supernatant. b Representative confocal microscopic images showing DCFH-DA fluorescence in BEAS-2B cells under control (CTRL) condition, or after treatment with SiNPs (100 μg/mL) or calcined-SiNPs (100 μg/mL). Scale bar = 50 μm. c Mean DCFH-DA fluorescence intensity under indicated conditions as shown in b, from 200 cells analyzed for each condition. d Representative confocal microscopic images showing DCFH-DA fluorescence in BEAS-2B cells under control (CTRL) condition or after treatment with SiNPs (100 μg/mL) in the absence or presence of DPI (0.1 μM). e. Mean DCFH-DA fluorescence intensity under indicated conditions as shown in d, from 200 cells analyzed for each condition. **P < 0.01, ***P < 0.001 compared to control group. #P < 0.05 compared to SiNPs-treated group. ΔP < 0.05, ΔΔP < 0.01 compared to calcined-SiNPs at the same concentration

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