Parameters | Day 1 | Day 4 | Day 7 | Day 14 | |
---|---|---|---|---|---|
BAL Cell Counts with 3.75 mg/100 g Body Weight Intra-tracheal (IT) Dose | |||||
 Macrophages (Cell Number per total lavage volume, × 106) | Talc | 5.5 ± 0.3 (NS) | 10 (NS) | 8 (NS) | 10 (S) |
Granite | 6.7 ± 1.0 (S) | 13 (S) | 13 (S) | 13 (S) | |
Control | 6.0 ± 0.5 | 6.5 | 6 | 6.2 | |
 Neutrophils (Cell Number per total lavage volume, ×106) | Talc | 11.7 ± 1.1 (S) | 4 (S) | 4 (S) | 4 (S) |
Granite | 20 ± 2.8 (S) | 15 (S) | 4 (S) | 0.8 (S) | |
Control | 1.5 ± 0.6 | 0.3 | 0.1 | 0.2 | |
BAL fluid Analyses with 3.75 mg/100 g Body Weight IT Dose | |||||
 Lactic Dehydrogenase (mU/ml Lavage fluid) | Talc | 139 ± 15 (S) | 80 (S) | 65 (NS) | 55 (S) |
Granite | 125 ± 7 (S) | 59 (S) | 43 (S2) | 37 (S2) | |
Control | 19 ± 1 | 22 | 25 | 21 | |
 Peroxidase (mU/ml Lavage fluid) | Talc | 210 ± 37 (S) | N/A | N/A | N/A |
Granite | 196 ± 36 (S) | N/A | N/A | N/A | |
Control | 2 ± 2 ± 0.3 | N/A | N/A | N/A | |
ß-N-Acetyl Glucosaminidase (mU/ml Lavage fluid) | Talc | 159 ± 9 (S) | 80 (S) | 82 (S) | 84 (S) |
Granite | 187 ± 21 (S) | 40 (S) | 35 (S) | 30 (S5) | |
Control | 25 ± 6 | 26 | 23 | 28 | |
 Albumin (μg/ml Lavage Fluid) | Talc | 1380 ± 116 (S) | N/A | N/A | N/A |
Granite | 2130 ± 262 (S) | N/A | N/A | N/A | |
Control | 66 ± 4 | N/A | N/A | N/A | |
In vivo Phagocytosis: Lambda assay uptake of labeled Particles after IT dust (3.75 mg/100 g Body Wt Dose) | |||||
 Lambda: Fraction of Radioactive Gold Particles Phagocytized | Talc | 0.46 ± 0.07 (S) | 0.56 (S) | 0.54 (S5) | 0.52 (S2) |
Granite | 0.47 ± 0.10 (S) | 0.85 (S5) | 0.82 (NS) | 0.7 (NS) | |
Control | 0.66 ± 0.3 | 0.75 | 0.73 | 0.78 |