Table 1 Parameters needed for dosimetric calculations with the 3DSDD model
From: NanoPASS: an easy-to-use user interface for nanoparticle dosimetry with the 3DSDD model
parameter | unit | commonly used methods | comments |
|---|---|---|---|
Nanoparticle characteristics | |||
hydrodynamic diameter or diffusion coefficient | [nm] or [nm2 s− 1] | NTA, DLS or NTA | One of the two parameters is needed, either as average or as whole distribution. |
effective density | [g cm− 3] | volumetric centrifugation method | The effective density of particles is the density of particle agglomerates that are formed for most particles in cell culture medium. For more detail please refer to [3]. |
Cell culture dish | |||
dish bottom area | [cm2] | length measured or manufacturer’s information | |
medium filling level | [cm] | height measured or calculated | |
Cell system | |||
height of cell growth at the walls | [cm] | height measured | Differentiated cell models like Caco-2 or HepaRG cells push their monolayer up the lateral wall of the culture dish during differentiation (see [5]). For undifferentiated cell models choose 0. |
Cell culture medium | |||
medium density | [g cm−3] | densitometer | |
medium viscosity | [mPa s] | viscometer | |
temperature during incubation | [°C] | thermometer | |
temperature during particle characterization | [°C] | thermometer | |
medium viscosity during particles characterization | [mPa s] | NTA | |
Calculation parameters | |||
number of particles simulated | – | – | Selecting more particles yields more detailed results, but increases the computational power needed (recommendation: 10,000 particles). |
simulation time | [h] | – | Corresponds to the maximal incubation time of interest. |
fraction of an hour a data snapshot is taken | [h] | – | These snapshots are needed for the interactive 3D-representation of the sedimentation process. This parameter defines the time that passes between two steps in the simulation process. |