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Fig. 5 | Particle and Fibre Toxicology

Fig. 5

From: Crystalline silica particles cause rapid NLRP3-dependent mitochondrial depolarization and DNA damage in airway epithelial cells

Fig. 5

Silica and FCCP rapidly induce NLRP3 recruitment to mitochondria in 16HBE cells. a, b Representative confocal images showing co-localization of Tom20 (red) and NLRP3 (green) in cells treated with silica (5 μg/cm2) (a) or FCCP (500 nM) (b) for times indicated. Bars show the co-localization as calculated by Pearson’s correlation coefficient. Data from 100 cells were analyzed. c PLA assay analysis for co-localization of NLRP3 and Tom20 in cells treated with silica (5 μg/cm2) for times indicated. Red dots indicate proximity between NLRP3 and Tom20. Nuclei were stained with DAPI (blue). Bars show means ± SD. All experiments were performed at least in triplicate. **p < 0.01 compared to untreated cells as determined by ANOVA

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