Fig. 4

Cytotoxicity assessment of incinerated thermoplastics in pSAECs. a-b Dose-response curves to graded depositional doses of incinerated thermoplastics reported by live-cell imaging and WST1 reduction for particle containing suspensions. c Interference-adjusted LDH release 24 and 48 h after exposure to depositional doses used in live-cell imaging assessments. d-e Representative images of JC-1-reported ΔΨm measurement 24 h after exposure; scale bars = 50 μm. As a comparison, 0.5 μM B[a]P served as a representative PAH to compare to particle treatments; 10 μM valinomycin served as a positive control for ΔΨm dissipation. e Quantitative metrics of images analyzed are presented with statistical comparisons. f-g pSAECs were assessed for CYP1 activity 48 h post exposure to incinerated thermoplastics and 0.5 μM B[a]P. Point estimates are the arithmetic mean of 2 independent experiments; error bars indicate standard error of the mean (SEM); *p < 0.05, **p < 0.01, compared to respective controls. Images taken at 20X